Fig. 5

Evaluation of invasiveness of cell clusters in vitro. A Time-lapse images of cell clusters in 3D gels. The yellow arrow indicates the direction of movement of the cell clusters. B Western blot analysis of the protein levels of vimentin and E-cadherin in MCF-7 cells. C Immunofluorescence analysis of the vimentin localization in MCF-7 cells. DAPI (nuclei, blue), F-actin(green), Vimentin (green). D, E Tumor cell-endothelial cell adhesion test. The lower layer is HUVEC cells, and the upper layer is MCF-7-GFP cells (green). The quantitated results, n = 5, ***P < 0.001. Two tail student's t-test analysis was used to compare the statistical difference between indicated two groups. F Cell- reattachment assay. The red arrow point to the cells that become fusiform