- Research article
- Open Access
- Open Peer Review
Early assessment by FDG-PET/CT of patients with advanced renal cell carcinoma treated with tyrosine kinase inhibitors is predictive of disease course
- Daiki Ueno1, 2,
- Masahiro Yao1, 3,
- Ukihide Tateishi4,
- Ryogo Minamimoto4,
- Kazuhide Makiyama1,
- Narihiko Hayashi1,
- Futoshi Sano1,
- Takayuki Murakami1,
- Takeshi Kishida5,
- Takeshi Miura5,
- Kazuki Kobayashi6,
- Sumio Noguchi6,
- Ichiro Ikeda7,
- Yoshiharu Ohgo2,
- Tomio Inoue3, 4,
- Yoshinobu Kubota1, 5 and
- Noboru Nakaigawa1, 5Email author
© Ueno et al.; licensee BioMed Central Ltd. 2012
- Received: 20 November 2011
- Accepted: 2 May 2012
- Published: 2 May 2012
We reported previously that 18F-2-fluoro-2-deoxyglucose positron emission tomography/ computed tomography (FDG PET/CT) had potential for evaluating early response to treatment by tyrosine kinase inhibitors (TKIs) in advanced renal cell carcinoma (RCC). This time we investigated the relation of the early assessment by FDG PET/CT to long-term prognosis with an expanded number of patients and period of observation.
Patients for whom TKI treatment for advanced RCC was planned were enrolled. FDG PET/CT was performed before TKI treatment and after one month of TKI treatment. The relations of the FDGPET/CT assessment to progression free survival (PFS) and overall survival (OS) were investigated.
Thirty-five patients were enrolled (sunitinib 19 cases, sorafenib 16 cases). The patients with RCC showing high SUVmax in pretreatment FDG PET/CT demonstrated short PFS (P =0.024, hazard ratio 1.137, 95% CI 1.017-1.271) and short OS (P =0.004, hazard ratio 1.210 95% CI 1.062-1.379). Thirty patients (sunitinib 16 cases, sorafenib 14 cases) were evaluated again after 1 month. The PFS of the patients whose SUVmax decreased<20% was shorter than that of the patients whose SUVmax decreased<20% (P = 0.027, hazard ratio 3.043, 95% CI 1.134-8.167). The PFS of patients whose tumor diameter sum increased was shorter than that of the patient with tumors whose diameter sum did not (P =0.006, hazard ratio 4.555, 95% CI 1.543-13.448).
The patients were classified into three response groups: good responder (diameter sum did not increase, and SUVmax decreased ≥ 20%), intermediate responder (diameter sum did not increase, and SUVmax decreased<20%), and poor responder (diameter sum increased, or one or more new lesions appeared). The median PFS of good, intermediate, and poor responders were 458 ± 146 days, 131 ± 9 days, and 88 ± 26 days (good vs. intermediate P = 0.0366, intermediate vs. poor P = 0.0097, log-rank test). Additionally the mean OSs were 999 ± 70 days, 469 ± 34 days, and 374 ± 125 days, respectively (good vs. intermediate P = 0.0385, intermediate vs. poor P = 0.0305, log-rank test).
The evaluation of RCC response to TKI by tumor size and FDG uptake using FDG PET/CT after 1 month can predict PFS and OS.
- Overall Survival
- Renal Cell Carcinoma
- Standardize Uptake Value
Renal cell carcinoma (RCC) accounts for 3% of all adult cancers . Approximately 30% of patients are diagnosed with metastases and an additional 20-40% of patients develop metastases after radical nephrectomy with curative intent [2, 3]. Cytokine therapies were the only systematic treatments available for advanced RCC for a long time, and the outcome of patients with metastatic RCC has been poor, with a median survival time of 10 to 21 months [4, 5].
Recently, the oncogenic mechanism of RCC has been elucidated and drugs that target relevant biological pathways have been developed. Tyrosine kinase inhibitors (TKIs) such as sunitinib and sorafenib which target vascular endothelial growth factor (VEGF) receptors improved the prognosis of patients with metastatic RCC [6, 7]. The antitumor activity of TKIs is not cytotoxic, like classical antitumor therapeutics, but rather cytostatic, suppressing biological activity by inhibiting angiogenesis. Practically, some RCCs treated with the TKIs do not decrease in tumor volume but enter a period of long-term dormancy, without enlargement of volume or novel metastasis. It has been suggested that a new assessment focusing not only on the volume of the tumors, but also biological activities to evaluate the antitumor activity of TKIs is necessary.
18F-2-fluoro-2-deoxyglucose positron emission tomography/ computed tomography (FDG PET/CT) is a useful non-invasive tool to evaluate glucose metabolic status, which can be the index of biological activity of cancer. Although PET has not been generally used for the screening of RCC due to the urinary excretion of the radiotracer, which can mask the presence of primary lesions [8, 9], several investigators have reported recently that FDG-PET/CT had sufficient potential to evaluate advanced RCCs [10–12]. We previously reported the potential of FDG PET/CT as a tool to evaluate the early response to TKIs in advanced RCC, but the number of cases was small and the observation period was short . This time, we investigated the potential with an expanded number of patients and period of follow-up.
Thirty-five patients were enrolled in this study. Patients had to be referred to Yokohama City University before the start of their treatment, from June 2008 until March 2011. This was a prospective study to clinically follow enrolled patients planning to undergo TKI therapy for advanced RCC. The pathologies of enrolled cases were confirmed by prior nephrectomy or biopsy. Patients with uncontrolled diabetes mellitus (blood glucose level >150 mg/dL) , with other known malignancies, and those treated with therapeutics during the 2 weeks prior to the scan were excluded. The study protocol was approved by the Yokohama City University Institutional Review Board. Written informed consent was obtained from all patients. The decision for patients to undergo therapy was made before the evaluation by PET/CT.
Sunitinib was given orally once a day at the dose of 50 mg in 6-week cycles consisting of 4 weeks of treatment followed by 2 weeks without treatment. Oral sorafenib 800 mg was given daily. The dose of sunitinib was reduced to 37.5 or 25 mg and that of sorafenib was reduced to 600 or 400 mg according to pretreatment general condition or major adverse events during treatment. Treatment was continued until disease progression, unacceptable adverse events, request by the patient, or surgery including nephrectomy.
Patients fasted for at least 6 hours prior to intravenous injection of 18F FDG. PET/CT images were obtained using a PET/CT system (Aquiduo 16; Toshiba Medical Systems, Tokyo, Japan). PET/CT images were acquired from the top of the head to the mid thigh at 60 min after intravenous injection of 2.5 MBq/kg of [18F] FDG. A low-dose non-contrasted CT scan was acquired first and used for attenuation correction. Emission images were acquired in 3-dimensional mode for 2 min per bed position. After PET acquisition, contrast-enhanced CT was performed with a 2-mm slice thickness, 120 kV, 400 mA, 0.5 s/tube rotation, from the top of the head to the mid thigh, with breath holding. A total of 100 ml contrast medium (iopamidol) was administered intravenously at a rate of 1.0 ml/s. The scan delay was set at 120 s after starting the injection of contrast material. The patients with serum creatinine level >1.5 mg/dL were examined without contrast material. Images were reconstructed by attenuation-weighted ordered-subset expectation maximization (OSEM) (four iterations, fourteen subsets, 128 ´ 128 matrix, with 5-mm Gaussian smoothing). The standardized uptake value (SUV) was determined according to the standard formula, with activity in the volume of interest (VOI) recorded as Bq per ml / injected dose in Bq per weight (kg). The maximum SUV (SUVmax) was recorded using the maximum pixel activity within the VOI. To obtain the SUVmax of the individual patient, the SUV of all lesions in tumors diagnosed as RCC by CT imaging were analyzed.
Cox proportional hazards model was used to assess the impacts of pretreatment SUVmax, SUVmax change ratio, total diameter change ratio, and clinical parameters on progression-free survival. The progression-free survival and overall survival curves were estimated by the Kaplan-Meier method, and the resulting curves were compared using the log-rank test.
The statistical difference of SUVmax and SUVmax change between clear cell carcinoma and papillary carcinoma was determined by two-side Mann-Whiteney’s U-test. All statistical analyses were carried out with SPSS software (SPSS, Inc, Chicago, IL). Significance was assigned at P<0.05.
Patients characteristics and intervention
Thirty-five patients were enrolled in this prospective study and evaluated by FDG PET/CT before treatment with TKIs (sunitinib 19 cases, sorafenib 16 cases). When the highest lesion SUV in individual patients was defined as SUVmax, SUVmax of the 35 patients ranged between 2.3 and 16.6 (mean 9.0). The patients with RCC tumors showing high SUVmax demonstrated short progression-free survival (PFS) (P = 0.024, hazard ratio 1.137, 95% CI 1.017-1.271) and short overall survival (OS) (P =0.004, hazard ratio 1.210 95% CI 1.062-1.379).
Characteristic of 30 patients
32-80 (mean 64)
Clinical outcome of 30 patients
The mean duration of observation was 458 days (range, 67–1114). At the date of analysis, 18 patients showed progressive disease (PD) as evaluated by RECIST version 1.1 and 10 patients had died due to progression of RCC. No patients had died for other reasons. The median PFS was 209 days (range, 27–887). Three patients (sunitinib, 2; sorafenib, 1) underwent nephrectomies after TKI treatment. Of the 14 patients treated with sorafenib, 10 patients changed to sunitinib due to PD, and 4 of the 10 patients changed to everolimus sequentially. One of the 14 sorafenib-treated patients changed directly to everolimus. Of the 16 patients treated with sunitinib, 4 patients changed to everolimus and 1 patient changed to sorafenib due to PD.
Univariate Cox progression-free survival analyses of various clinical parameters
sunitinib vs. sorafenib
clear cell vs. papillary
nephrectomy: yes vs. no
pretreatment: yes vs. no
previous TKI: yes vs. no
previous IFN: yes vs. no
number of lesions: 1–2 vs. 3 ≥
lung metastasis: only vs. others
bone metastasis: no vs. yes
liver metastasis: no vs. yes
The assessment by FDG PET/CT
In pretreatment FDG PET/CT of the 30 patients who underwent two-time assessment, FDG accumulation was detected in 95 lesions of 107 lesions (89%) whose diameters were 1.0 cm or more. The mean number of RCC lesions in the individual patients was 3.5 (range, 1–9). The median date of the second FDG PET/CT after TKI treatment started was day 31 (range, 27–47). The median SUVmax in the second FDG PET/CT was 7.1 (range, 3.7-15.5). The mean ratio of SUVmax change compared with pretreatment FDG PET/CT was −18% (range, -55 to 65%). The mean ratio of the diameter change was −6% (range, -30 to 30%). No lesion remitted completely. A new lesion appeared in only 1 patient. The mean ratio of SUVmax change in clear cell carcinoma was −14.0%(range, -54.9%- 65.2%), and that in papillary carcinoma was −1.1%(range, -35.4%- 15.7%). The mean ratio of the diameter in in clear cell carcinoma was −5.7%(range, -30.2%- 29.7%), and that in papillary carcinoma was −6.5%(range, -22.4%- 13.8%). The ratios of SUVmax change and diameter change were not statistically different between clear cell carcinoma and papillary carcinoma (SUVmax change: p = 0.193, diameter change: p = 0.954).
According to the European Organization for Research and Treatment of Cancer (EORTC) criteria , in which the SUV cut-off point is 25%, 9 patients had a partial metabolic response, 14 patients had SD, and 7 had PD. None achieved complete remission (CR). There was no statistical association between the evaluation by EORTC criteria and PFS. However, the PFS of the patients whose tumor SUVmax decreased<20% after 1 month was shorter than that of those whose tumor SUVmax decreased<20% (P = 0.027, Cox hazard ratio 3.043, 95% CI 1.134-8.167). Additionally, the PFS of patients whose tumor diameter sum increased after 1 month was shorter than that of the patients whose tumors diameter sum did not increase (P = 0.006, hazard ratio 4.555, 95% CI 1.543-13.448).
Using these two predictive factors, we defined new and simple criteria for evaluating tumor response to TKI of advanced RCC as follows: good responder, diameter sum does not increase and SUVmax decreases<20%; intermediate responder, diameter sum does not increase and SUVmax decreases<20%; and poor responder, diameter sum increases or one or more new lesions appear.
However, it was not sufficient to assess the response to TKIs of RCCs by FDG uptake alone because there were some RCC cases with decreased SUVmax but increased tumor volume, and their prognoses were poor as shown Figure 1. In our series, 3 patients demonstrated a decrease in SUVmax ≥ 20% but an increase in tumor size after 1 month, and their PFSs were very short: 28, 28, and 146 days, respectively. Additionally, the classification of our series by EORTC criteria, which assess the response by FDG uptake, was not statistically associated with prognosis. Kayani et al. previously reported that the assessment of patients with RCC treated with sunitinib by FDG uptake alone after 4 weeks failed to predict the disease course . These results suggested the existence of RCCs that progress independently of glucose uptake, and emphasize the need for a combination assessment of both tumor size and FDG uptake.
Some questions remain. We defined metabolic response as a ≥20% reduction of SUVmax in the novel criteria we advocated. The EORTC proposed a SUV diminution of 15-25% for a partial response after the first cycle. Kayani et al. the defined cut-off point as 20% when they evaluated the response to sunitinib in metastatic RCC and succeeded in predicting the disease course by assessment after 16 weeks. Lyrdal et al. reported that responders with a decrease of ≥20% FDG accumulation to sorafenib had a long OS. A further study expanding the number of patients is necessary to verify this cut-off point.
The evaluation of RCC response to TKI treatment by tumor size and FDG uptake using FDG PET/CT after 1 month can predict not only the duration of response to TKIs, but also survival duration. Early assessment by FDG-PET/CT provides useful information to determine strategies for individual patients with advanced RCC. However, larger studies are needed to confirm these preliminary results.
This works was supported by Grant for Research and Development Project II (No. 17) of Yokohama City University, Japan and “ Establishment of research center for clinical proteomics of post-translational modifications” as part of the Special Coordination Fund for Promoting Science and Technology “Creation and Innovation Centers for Advanced Interdisciplinary ”, and Grants-in-Aid for Scientific Research (No. 22591775) from the Ministry of Education, Science, Sports and Culture of Japan.
- Chow WH, Devesa SS, Warren JL, Fraumeni JF: Rising incidence of renal cell carcinoma in the United States. JAMA. 1999, 281: 1628-1631. 10.1001/jama.281.17.1628.View ArticlePubMedGoogle Scholar
- Linehan WM, Walther MM, Alexander RB, Rosenberg SA: Adoptive immunotherapy of renal cell carcinoma: studies from the Surgery Branch, National Cancer Institute. Semin Urol. 1993, 11: 41-43.PubMedGoogle Scholar
- Janzen NK, Kim HL, Figlin RA, Belldegrun AS: Surveillance after radical or partial nephrectomy for localized renal cell carcinoma and management of recurrent disease. Urol Clin North Am. 2003, 30: 843-852. 10.1016/S0094-0143(03)00056-9.View ArticlePubMedGoogle Scholar
- Motzer RJ, Bander NH, Nanus DM: Renal-cell carcinoma. N Engl J Med. 1996, 335: 865-875. 10.1056/NEJM199609193351207.View ArticlePubMedGoogle Scholar
- Naito S, Yamamoto N, Takayama T, Muramoto M, Shinohara N, Nishiyama K, Takahashi A, Maruyama R, Saika T, Hoshi S, Nagao K, Yamamoto S, Sugimura I, Uemura H, Koga S, Takahashi M, Ito F, Ozono S, Terachi T, Naito S, Tomita Y: Prognosis of Japanese Metastatic Renal Cell Carcinoma Patients in the Cytokine Era: A Cooperative Group Report of 1463 Patients. Eur Urol. 2009, 57: 317-326.View ArticlePubMedGoogle Scholar
- Motzer RJ, Hutson TE, Tomczak P, Michaelson MD, Bukowski RM, Rixe O, Oudard S, Negrier S, Szczylik C, Kim ST, Chen I, Bycott PW, Baum CM, Figlin RA: Sunitinib versus interferon alfa in metastatic renal cell carcinoma. N Engl J Med. 2007, 356: 115-124. 10.1056/NEJMoa065044.View ArticlePubMedGoogle Scholar
- Escudier B, Eisen T, Stadler WM, Szczylik C, Oudard S, Siebels M, Negrier S, Chevreau C, Solska E, Desai AA, Rolland F, Demkow T, Hutson TE, Gore M, Freeman S, Schwartz B, Shan M, Simantov R, Bukowski RM, TARGET Study Group: Sorafenib in advanced clear cell renal cell carcinoma. N Engl J Med. 2007, 356: 125-134. 10.1056/NEJMoa060655.View ArticlePubMedGoogle Scholar
- Aide N, Cappele O, Bottet P, Bensadoun H, Regeasse A, Comoz F, Sobrio F, Bouvard G, Agostini D: Efficiency of [(18)F]FDG PET in characterising renal cancer and detecting distant metastases: a comparison with CT. Eur J Nucl Med Mol Imaging. 2003, 30: 1236-1245. 10.1007/s00259-003-1211-4.View ArticlePubMedGoogle Scholar
- Kang DE, White RL, Zuger JH, Sasser HC, Teigland CM: Clinical use of fluorodeoxyglucose F 18 positron emission tomography for detection of renal cell carcinoma. J Urol. 2004, 171: 1806-1809. 10.1097/01.ju.0000120241.50061.e4.View ArticlePubMedGoogle Scholar
- Majhail NS, Urbain JL, Albani JM, Kanvinde MH, Rice TW, Novick AC, Mekhail TM, Olencki TE, Elson P, Bukowski RM: F-18 fluorodeoxyglucose positron emission tomography in the evaluation of distant metastases from renal cell carcinoma. J Clin Oncol. 2003, 21: 3995-4000. 10.1200/JCO.2003.04.073.View ArticlePubMedGoogle Scholar
- Park JW, Jo MK, Lee HM: Significance of 18F-fluorodeoxyglucose positron-emission tomography/computed tomography for the postoperative surveillance of advanced renal cell carcinoma. BJU Int. 2009, 103: 615-619. 10.1111/j.1464-410X.2008.08150.x.View ArticlePubMedGoogle Scholar
- Namura K, Minamimoto R, Yao M, Makiyama K, Murakami T, Sano F, Hayashi N, Tateishi U, Ishigaki H, Kishida T, Miura T, Kobayashi K, Noguchi S, Inoue T, Kubota Y, Nakaigawa N: Impact of maximum standardized uptake value (SUVmax) evaluated by 18-Fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography (18F-FDG-PET/CT) on survival for patients with advanced renal cell carcinoma: a preliminary report. BMC Cancer. 2010, 10: 667-10.1186/1471-2407-10-667.View ArticlePubMedPubMed CentralGoogle Scholar
- Minamimoto R, Nakaigawa N, Tateishi U, Suzuki A, Shizukuishi K, Kishida T, Miura T, Makiyama K, Yao M, Kubota Y, Inoue T: Evaluation of response to multikinase inhibitor in metastatic renal cell carcinoma by FDG PET/contrast-enhnced CT. Clin Nucl Med. 2010, 35 (12): 918-923. 10.1097/RLU.0b013e3181f9ddd9.View ArticlePubMedGoogle Scholar
- Motzer RJ, Bacik J, Schwartz LH, Reuter V, Russo P, Marion S, Mazumdar M: Prognostic factors for survival in previously treated patients with metastatic renal cell carcinoma. J Clin Oncol. 2004, 22 (3): 454-463.View ArticlePubMedGoogle Scholar
- Young H, Baum R, Cremerius U, Herholz K, Hoekstra O, Lammertsma AA, Pruim J, Price P: Measurement of clinical and subclinical tumour response using [18F]-fluorodeoxyglucose and positron emission tomography: review and 1999 EORTC recommendations. European Organization for Research and Treatment of Cancer (EORTC) PET Study Group. Eur J Cancer. 1999, 35 (13): 1773-1782. 10.1016/S0959-8049(99)00229-4.View ArticlePubMedGoogle Scholar
- Hudes G, Carducci M, Tomczak P, Dutcher J, Figlin R, Kapoor A, Staroslawska E, Sosman J, McDermott D, Bodrogi I, Kovacevic Z, Lesovoy V, Schmidt-Wolf IG, Barbarash O, Gokmen E, O'Toole T, Lustgarten S, Moore L, Motzer RJ, ARCC Global Trial: Temsirolimus, interferon alfa, or both for advanced renal-cell carcinoma. N Engl J Med. 2007, 356 (22): 2271-2281. 10.1056/NEJMoa066838.View ArticlePubMedGoogle Scholar
- Motzer RJ, Escudier B, Oudard S, Hutson TE, Porta C, Bracarda S, Grünwald V, Thompson JA, Figlin RA, Hollaender N, Urbanowitz G, Berg WJ, Kay A, Lebwohl D, Ravaud A, RECORD-1 Study Group: Efficacy of everolimus in advanced renal cell carcinoma: a double-blind, randomised, placebo-controlled phase III trial. Lancet. 2008, 372 (9637): 449-456. 10.1016/S0140-6736(08)61039-9. Epub 2008 Jul 22View ArticlePubMedGoogle Scholar
- Krajewski KM, Guo M, Van den Abbeele AD, Yap J, Ramaiya N, Jagannathan J, Heng DY, Atkins MB, McDermott DF, Schutz FA, Pedrosa I, Choueiri TK: Comparison of four early posttherapy imaging changes (EPTIC; RECIST 1.0, tumor shrinkage, computed tomography tumor density, Choi criteria) in assessing outcome to vascular endothelial growth factor-targeted therapy in patients with advanced renal cell carcinoma. Eur Urol. 2011, 59 (5): 856-862. 10.1016/j.eururo.2011.01.038.View ArticlePubMedGoogle Scholar
- Fournier LS, Oudard S, Thiam R, Trinquart L, Banu E, Medioni J, Balvay D, Chatellier G, Frija G, Cuenod CA: Metastatic renal carcinoma: evaluation of antiangiogenic therapy with dynamic contrast-enhanced CT. Radiology. 2010, 256 (2): 511-518. 10.1148/radiol.10091362. Epub 2010 Jun 15View ArticlePubMedGoogle Scholar
- Kayani I, Avril N, Bomanji J, Chowdhury S, Rockall A, Sahdev A, Nathan P, Wilson P, Shamash J, Sharpe K, Lim L, Dickson J, Ell P, Reynolds A, Powles T: Sequential FDG-PET/CT as a Biomarker of Response to Sunitinib in Metastatic Clear Cell Renal Cancer. Clin Cancer Res. 2011, 17: 6021-6028. 10.1158/1078-0432.CCR-10-3309.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://0-www.biomedcentral.com.brum.beds.ac.uk/1471-2407/12/162/prepub
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