Figure 3From: High expression of KPNA2 defines poor prognosis in patients with upper tract urothelial carcinoma treated with radical nephroureterectomyKPNA2 knockdown induces apoptosis in urothelial carcinoma cells. (A,B) The apoptosis rate, as evaluated by flow cytometry analysis using Annexin-V/PI staining. (C,D) The percentage of cells with mitochondrial Δψm disruption, as evaluated by flow cytometry analysis using the JC-1 dye. (E) Caspase 3/7 activity of T24 and J82 cells transfected with control or KPNA2-directed siRNA. The data in (A–E) represent the mean ± SD of three independent tests performed in triplicate. *P < 0.05. (F) Western blot analysis of PARP cleavage and PCNA expression in control and KPNA2 knockdown cells.Back to article page